Wash Buffer Concentrate was diluted 10-fold. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Modethe introduction of the SPECTRAmax PLUS microplate spectrophotometer and its PathCheck feature, the ability to determine the pathlength in each well of a microplate and automatically normalize the absorbance value to a 1 cm pathlength. • Tel. Filterless readers provide consistent results with steady temperature regulation. ) injection of 3 mLThe fluorescence intensity was detected by using a SpectraMax M5 microplate reader from Molecular Devices Co. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 05% Tween 20 using a Nunc ELISA plate washer. 6-384 (96- and 384-well with pipetting) 6-384. Other technologies such as imaging. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 2. You should get comparable results from top and bottom reads using EC50's to compare reads. Sale price $18,499. 26 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Microplate Readers include the M3, M4, M5, and M5e models. The assay window spanned four orders of magnitude. 0 and 0. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Molecular Devices Spectramax M5 Microplate Reader. Bookings are free, but you will need to use iLab to book. M3-M5e_manual. 1. es •. Five preferred detection modes. FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax® M5 Multi-Mode Microplate Reader (cat. 00 USD. For the Live/Dead assay, they were treated. Label-free detection. This reader's dual monochromators for. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. SpectraMax® iD5. 87. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Call 516-939-0888 to check. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 6 for further experiment, so I'm confused which i would choose for OD value determination. Cavicide (Warehouse, Cat # 79300360). Here the delta mP was 336, and Z’ factor was 0. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Go to Product Details. Molecular Devices. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Italic and boldface type are used for emphasis. E Models Only) 69. D. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Microplate Readers include the M3, M4,. FlexStation™ I. 04 to 3. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The advantage of using labeled probes is detection specificity. Page 28 Chapter 5: Read Modes and Read Types 1. Qualify the fluorescence performance of the SpectraMax . 5. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). 5°C at 37°C. Cell viability was quantified by measuring the absorbance of the solution at 570 nm using a SpectraMax® M5 microplate reader (Molecular Devices). Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). l. 1. The CellTiter-Glo assay from Promega uses luciferase. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 0 out of 5 $ 3,799. Catalog Number: (89212-396) Supplier: Molecular Devices. A microplate reader asThe SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Please refer to the Obsolete Microplate Reader Models for more information. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Wide range of applications. A: Microplate Preparation. The samples were boiled for 5 min with 1 ×. free tracer decreases, resulting in. With optimized reagents, option to operate the. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Equipped with scanning, shaking and kinetics modules. Molecular Devices SpectraMax® series microplate readers. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. Protocol Name Version 6. Fluorescence Microplate Readers. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Category 2 and 3 microplate readers use gradient heating as well. 2. We would like to show you a description here but the site won’t allow us. #SpectraMax L Config) Methods TIGIT/CD155 Blockade Bioassay. Physical specifications and backup power UPS Choices for SpectraMax® and Gemini™ microplate readers. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Cuvette port functionality can also be controlled using SoftMax Pro Software. 6: bioNova científica, s. The assay window spanned four orders of magnitude. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. . The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Page 1 of 8 . Western blotting assays were conducted according to previous studies . 95. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 1** Version 7. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. The cells were incubated with compounds at the indicated doses for 1 h. FlexStation™ III. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. 59 6. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. The spectrophotometric. Or scan up to 384 wells at any time to determine the best assay conditions. These lab workhorses are used in drug discovery, research, bioassay validation, and. Plate chamber design of the SpectraMax category 1 microplate readers. Published Dec 16, 2020 | Updated Dec 16, 2020. Levels of immunoreactivity were normalized to. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. The assay applies fluorescence polarization to quantify Fc-containing IgG. 312 gain calibration failed. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Five. Fifteen different automated tests are provided. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. 314 low lamp level warning. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 1. 4. Fluorescence Microplate Reader Comparison Chart. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. This application note describes how to. At the end of this period, cells were exposed to FA (1 mM) for 1 h. Thank you!. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. Figure 1. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. book Page 7 Wednesday, July 7, 2010 2:09 PM. 63 1. KEY FEATURES • Five. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. The SpectraMax® ABS Plus Microplate Reader can run . 1. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. com. 2. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Fluorescence Microplate Readers. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 0 μL) instead of the sample solution was applied as control group, and. 54 1. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideFluorescence Microplate Reader Comparison Chart. The acceptance. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Western blotting analysis. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. 00 USD. The Spectr. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Add to Cart. SpectraMax iD5 Multi-Mode Microplate Reader. TR-FRET assay. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Instrument settings are shown in Figure 3. 0 OD for earlier models. 00. We use Spectramax M5 microplate reader in our lab. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax M2 and M2e are versatile and reliable. ATP standard curves read on SpectraMax microplate readers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Expanded wavelength coverage. Label: provides information about the reader, such as line voltage rating, caution-ary information, serial number, etc. Has anyone had problems with the built-in computer of the Spectramax ID3. B: Selection of Excitation Wavelength. The concentration of antibodies was determined by using a spectrophotometer from Puxi General Instrument Co. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Microplate Readers are modular and . The system. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Detailed specifications of each model reader are reported in Section 7, “Specifications”. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. 43 Max %CV 1. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 4 0. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Parallel negative control assays had primary, secondary, or both antibodies omitted. reader in the visible range between 405–750 nm with NIST and NMI. 2. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. HTRF-compatible SpectraMax Multi-Mode Readers. The CellTiter-Glo assay from Promega uses luciferase. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 1 is GxP edition only; Protocols for the ABS1 Validation Plate. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. 7 cells were co-transfected with 0. analysis, controls the SpectraMax Multi-Mode Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. for ValitaTiter Plus standards read on Molecular Devices readers. book Page 7 Wednesday, July 7, 2010 2:09 PM. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Here the delta mP was 336, and Z’ factor was 0. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. 4. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) M5 SpectraMax M5 e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5 e models. IMAP assays are based on binding of. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. Access this by going to Help >. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. PURPOSE. Both readers feature an 8-channel read head for fast reads and. Spectramax M5 microplate reader: Molecular Devices: N/A:. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Most papers they cultured bacteria to OD600>0. Pick the best speed, sensitivity, and price for your needs. The following document is. 2. It cannot be used for luminescence measurements. , USA). Figure 1. 72 MB. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown menu. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. TR-FRET assay. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Microplate Readers are modular and . Choose from a. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Selection of Water Constant in PathCheck settings. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. Our absorbance plate readers feature our PathCheck Sensor. 7. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. ® reader to the computer. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. Standard features include a. Microplate Readers include the M3, M4, M5, and M5e models. Molecular Devices Spectramax M5 Microplate Reader. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 1. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. Also refer to this Multimode Reader Comparison Chart . com. Bookings are free, but you will need to use iLab to book. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). The cuvette must be in place when you read the plate. SpectraMax Glo Steady-Luc Reporter Assay Kit. Lift up the lid over the chamber to insert or remove a cuvette. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM SpectraMax M5 Microplate Reader: Read type = endpoint, Read Mode = luminescence with 1000 ms integration time, Wavelengths = All, Automix = off, Auto calibration = on, Settling time = off, Autoread = off. When the user applies a set temperature of 37°C, the top is heated to 37. The SpectraMax 190 reader can be integrated with Molecular . TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Category 2 and 3 microplate readers use gradient heating as well. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Figure 4: Transcreener ADP. Maximum signal with lowest background. Ltd. SDS-PAGE Assay. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. 00 USD. Add to Cart. The SpectraMax M2 and M2e are versatile and reliable. Ferguson, Ph. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The following document is intended to demonstrate setup of this instrument. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. The dual monochromator optics allow. The SpectraMax® iD3 Multi Mode Microplate . Molecular Devices. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. To block unwanted residual excitation light and minimize background interference, a 570 nm. 7°C and the bottom to 36. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. As. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. 6. The raw data from each individual experiment were normalized to vehicle-treated HK-2. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. Ltd. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Microplate Readers. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. These robust readers have been placed in labs from Antarctica to. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. Molecular Devices Spectramax M5 Microplate Reader. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. 0 out of. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. SpectraMax® M4. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. M3-M5e_manual. Example: [Drawer]. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. Five preferred modes for diverse bioresearch applications. Spectramax Multi-Mode Microplate Reader Performance Specifications. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. 1. After being incubated in the dark at 60 ℃ overnight, the supernatants were centrifuged and optical densities were measured at 750, 665, 652 and 470 nm using the SpectraMax M5 microplate reader (Molecular Devices,. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. When the user applies a set temperature of 37°C, the top is heated to 37. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. 5 Dual-luciferase reporter assay. 0 . After that, the cells were resuspended and diluted to a suitable density using DCFH-DA working solution, transferred to an opaque 96-well plate and incubated for 30 min. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7.